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Search Result For ' Immunohistochemistry' , Result Number : 6

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Tayseer Refaat Ibrahim Elsayed
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
Clinical Significance of Serum Galectin-1 and Its Tissue Immunohistochemical Expression in Serous Ovarian Carcinoma Patients Mai M. Abdelwahab, Huda F. Ebain , Taiseer R. Ibrahim, Mohamed S. Badr, Mohamed E. Lashin, Mahamoud Abdou Yassin, Adel M. Ismail, Ahmed A. Obaya Abstract Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type with poor prognosis due to early metastasis and first presentation with advanced stage. In this work, we investigated serum level of Galac-tin-1 (Gal-1) and its tissue immunohistochemical expression in SOC patients at different stages trying to find out its significance as a diagnostic and prognostic marker. Patients and methods: The study included 95 females I-Control group: Twenty five healthy females; II-Patients group: Seventy females diagnosed as SOC at different stages; Stage I: 8 cases, Stage II: 12 cases, Stage III: 32 cases and Stage VI:18 cases. Serum Galectin-1 and CA-125 were measured by ELIZA and tissue Galectin-1 was assessed by immunohistochemistry. All patients were followed for up to 3 years after surgery. Results: Serum Gal-1 and CA-125 levels were significantly high in SOC patients compared to controls (p < 0.001). We found a direct positive statistically significant correlation between serum Gal-1 and CA125 levels (p < 0.001). Se-rum Gal-1 at cut off value > 135 ng/ml was superior to CA-125 a cut off value > 49 u/ml with sensitivity, specificity of 100%, vs 88.57, 96% for CA-125. Serum Gal-1 was significantly associated with tumor stage (p < 0.001). Immunohistochemistry showed that patients with strong Gal-1 expression had higher serum level (p = 0.002). Stromal and tumor Gal-1 expression were significantly correlated with tumor grade (p < 0.001) and stage (p = 0.001). Se-rum Gal-1, CA-125 and IHC Gal-1 expression were associated with poor survival (p < 0.001, p = 0.009 and p = 0.002) respectively. Conclusion: Serum Gal-1 and its tissue IHC expression are useful diagnostic and prognostic markers for SOC patients.
Raafat Awad Mostafa
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
Immunohistochemistry
Samah Saied Abdelwahab
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
Prognostic significance of immunohistochemistry and proliferative activity in colorectal cancer using Survivin, COX2, S phase fraction and DNA ploidy.
Nadya ebasiry Elsayed
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
ABSTRACT Background: Hyperglycemia associated with the diabetes mellitus (DM) commonly results in structural abnormalities and hyposalivation in the parotid glands. An antioxidant action in a potent multivitamin called Antox (ANX) aids in reducing oxidative stress. Aim of study: The current study examined the role of ANX in preventing complications from diabetes in the parotid gland, as well as its potential mechanisms. Material and methods: 36 rats were randomly divided into six groups; each group contained six rats. Group 1 (Negative (-ve) control): the rats received only regular food and water. Group 2 (vehicle group): rats received a single i.p. dose of citrate buffer as a vehicle. Group 3: rats were given ANX by oral gavage with a polyethylene canula 0.5 mm in a volume not to exceed 0.3 ml/100 gm at a dose of 10 mg/kg/day. Group 4 (Diabetic/ DM): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer. Group 5 (DM+ Insulin): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer and insulin (Mixtard 30/70; Novo Nordisk) 1 U/100 gm once daily subcutaneous (S.C.). Group 6 (DM+ Insulin + ANX): rats received a single i.p. dose of 50 mg/kg of STZ and insulin 1 U/100 gm /day/S.C. then received ANX orally in a dose of 10 mg/kg/day. All medications were given for 4 weeks. Rats were anaesthetized, and the parotid tissues were obtained for biochemical analysis to measure Malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and nitric oxide (NO), for Histopathological examination (Hematoxylin and Eosin staining, Masson trichrome stain) and immunohistochemistry using (8-OHdG, α-SMA and BAX). Results: Remarkably, co-administration of ANX with insulin significantly reduced malondialdehyde (MDA) levels and increased expression of 8-hydroxy-2'-deoxyguanosine (8-OHdG) while enhancing the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC). Additionally, compared to the diabetic rat group and the diabetic group receiving insulin, the combination of ANX and insulin resulted in a considerably decreased expression of Bcl-2, Associated X-protein (Bax) and Smooth muscle alphaactin (α-SMA), with significant reduction in interleukin-1beta (IL1β) level in relation to diabetic group. Conclusion: In comparison to insulin administration alone, co-delivery of ANX with insulin throughout diabetes treatment was more effective in preserving the structure and function of the parotid glandHISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY ON THE POSSIBLE AMELIORATING EFFECTS OF ANTOX ON THE PAROTID GLAND OF RATS WITH STREPTOZOTOCIN-INDUCED DIABETES MELLITUS TYPE 1
Nadya ebasiry Elsayed
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
Background: The cerebellum is the second largest part of the mammalian brain. It is a key regulator of coordinated sensory and musculoskeletal actions. The cerebellar cortex has a striking morphology consisting of folia and fissures and a variety of cells which are morphologically and functionally different, so it’s considered as an ideal model to study the development of the central nervous system in the mammals. The Objectives: The aim of this work was to study the prenatal and postnatal development of the albino rat cerebellar cortex considering its structure and maturation. Material And Methods: 35 healthy, non-pregnant female and 18 male albino rats weighing (200-250 g) were obtained from the animal house, Faculty of Medicine, Zagazig University. After mating and pregnancy, the rat embryos and offsprings were divided into 3 groups and 6 subgroups; Group A (11th, 16th&20th fetal rats), Group B (7th, 14th &21st postnatal days rats) and Group C (3 months old rats). The fetal rats (11th and 16th) were fixed as whole, while the remaining prenatal, postnatal and adult rats were dissected to obtain cerebella, which were processed for light and electron microscopic examinations, morphometric and immunohistochemical studies. Results: Albino rats at prenatal day 11 (E11) showed formation of the neural tube from the ectodermal layer. Albino rats at prenatal day 16 (E16) showed a demarcated cerebellar anlage (rhombic lip ( at the dorsal part of the metencephalon. The cerebellar cortex at prenatal day 20 (E 20) showed appearance of the general architecture. From outwards to inwards, it consisted of external granular, molecular, Purkinje cell, internal granular layers. Thickness of the external granular layer was maximum at postnatal day 7 (D7) then gradually decreased with age till disappearance in adult age. The molecular layer showed a gradual increase in thickness with age till reaching its maximum size at adult stage. Purkinje cells at (E20) and (D7) were not arranged in a definite layer. It appeared as a single layer between the molecular and internal granular layer at D14, D21 and adult. The internal granular was consisted of scattered small granule cells at E20 and D7. At D14 the internal granular layer became well differentiated and distinguished from the white matter. Immunohistochemistry showed a negative reaction for glial fibrillary acidic protein (GFAP) at E11 and E16. The first positive reaction for GFAP appeared at E20 then gradually increased till adult group. Conclusion: The study concluded that the cerebellar cortex undergoes differentiation and maturation during late prenatal and early postnatal ages early postnatal ages till the third week of life, corresponding to nearly the seventh postnatal month in human. Therefore the development of the cerebellum is very critical and sensitive during these periods makes it susceptible to malformationPRENATAL AND POSTNATAL DEVELOPMENTAL CHANGES OF THE CEREBELLAR CORTEX IN ALBINO RATS
Nadya ebasiry Elsayed
- Faculty Research Area On Zu Site
- Faculty Research Area On Staff Site
International Immunopharmacology 2023; Volume 115: 109621 Background Ulcerative colitis (UC) is a global inflammatory bowel disease. Aime :This study aimed to assess the effects of icosapent ethyl on acetic acid-induced colitis in rats as well as the underlying mechanisms involved. Methods 36 male Wister rats were equally divided into Six groups: control, UC, mesalamine 100 mg/kg, icosapent 150mg/kg, icosapent 300 mg/kg, and EX527-icosapent 300 mg/kg groups. EXcept for control group, UC was induced by acetic acid instillation into colon. Drugs were administered once daily for one week then under thiopental anaesthesia, colons were excised. Colitis macroscopic and microscopic scores were assessed. A part of colon was homogenized for detection of malondialdehyde (MDA), inerleukin1 (IL-1β), tumor necrosis factor (TNF-α), superoxide dis-mutase (SOD), phosphorylated Akt (pAkt) and caspase 3 levels. Silent information regulator 1 (SIRT1), hemeoxygenase 1 (HO-1), and nuclear factor erythroid 2 (Nrf2) mRNA expressions were detected. Mallory-stained colonic sections were examined for collagen fibres detection. Immunohistochemistry of NF-κB and p53 expressions in colonic sections were assessed. Results There acetic acid induced colitis increments in MDA, IL-1β, TNF-α, and caspase 3 levels while decreased SOD, pAkt, SIRT1, HO-1, and Nrf2 with increased collagen fibres as well as NF- κB and p53. Icosapent decreased macro& microscopic colitis scores, MDA, IL-1β, TNF-α, and caspase 3 levels while increased SOD, pAkt, SIRT1, HO-1, and Nrf2 with decreased collagen fibres as well as NF-κB and p53. The effects of icosapent 300 mg/kg were similar to mesalamine. Icosapent effects were antagonized by EX527. Conclusion Icosapent alleviated acetic acid-induced colitis via its anti-inflammatory, Antioxidant, and anti-apoptotic effects mediated in part by SIRT1 pathway activationIcosapent ethyl alleviates acetic acid-induced ulcerative colitis via modulation of SIRT1 signaling pathway in rats